Validation Data Gallery
Tested Applications
This antibody is equivalent to Ser555 in mice.
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
80218-1-PBS targets Phospho-ULK1 (Ser556) in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | unc-51-like kinase 1 (C. elegans) |
| Observed molecular weight | 140 kDa |
| GenBank accession number | NM_003565 |
| Gene Symbol | ULK1 |
| Gene ID (NCBI) | 8408 |
| RRID | AB_2918877 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75385 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367)