Phospho-ULK1 (Ser556) Recombinant antibody
Phospho-ULK1 (Ser556) Recombinant Antibody for WB, ELISA, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (3)
Applications
WB, IHC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
2F5
Cat no : 80218-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells |
| Positive FC (Intra) detected in | Calyculin A treated PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 15 publications below |
| IHC | See 1 publications below |
Product Information
80218-1-RR targets Phospho-ULK1 (Ser556) in WB, IHC, FC (Intra), ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | unc-51-like kinase 1 (C. elegans) |
| Observed molecular weight | 140 kDa |
| GenBank accession number | NM_003565 |
| Gene symbol | ULK1 |
| Gene ID (NCBI) | 8408 |
| RRID | AB_2918877 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-ULK1 (Ser556) antibody 80218-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hazard Mater Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells. | ||
JCI Insight ZNFX1 promotes AMPK-mediated autophagy against Mycobacterium tuberculosis by stabilizing mRNA | ||
Bioengineered Shikonin induces apoptosis and autophagy via downregulation of pyrroline-5-carboxylate reductase1 in hepatocellular carcinoma cells. | ||
Oxid Med Cell Longev tRNA-Derived Fragment tRF-5009A Regulates Autophagy and Degeneration of Cartilage in Osteoarthritis via Targeting mTOR | ||
Cell Death Dis IKCa channels control breast cancer metabolism including AMPK-driven autophagy | ||
Ecotoxicol Environ Saf Role of SESTRIN2/AMPK/ULK1 pathway activation and lysosomes dysfunction in NaAsO2-induced liver injury under oxidative stress |
