Validation Data Gallery
Tested Applications
This site is also equivalent to Ser77 for c-MYC isoform 2 (P01106-2).
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
28915-1-PBS targets Phospho-c-MYC (Ser62) in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | v-myc myelocytomatosis viral oncogene homolog (avian) |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC000141 |
| Gene Symbol | MYC |
| Gene ID (NCBI) | 4609 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01106 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
MYC is a transcription factor that globally enhances expression of transcribing genes, including those vital for cell cycle, growth, proliferation, and survival in normal and cancer cells. MYC has multiple isomers, and this antibody recognizes the phosphorylation of the 439 amino acid isomer (P01106-1, UniProt) at the 62 serine site. Posttranslational modifications that regulate MYC stability include phosphorylations at Ser62 and Thr58. Phosphorylation at Ser62, primarily by ERK, stabilizes MYC. While kinases other than ERK phosphorylate Ser62, GSK3β has been the only kinase known to phosphorylate MYC at Thr58.(PMID: 32482868)