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CoraLite® Plus 488-conjugated Prohibitin 2 Monoclonal antibody
Prohibitin 2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1D9C7
Cat no : CL488-66424
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66424 targets Prohibitin 2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Prohibitin 2 fusion protein Ag2977 相同性解析による交差性が予測される生物種 |
| Full Name | prohibitin 2 |
| Calculated molecular weight | 299 aa, 33 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC014766 |
| Gene symbol | Prohibitin 2 |
| Gene ID (NCBI) | 11331 |
| RRID | AB_2883335 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Prohibitin 2 antibody CL488-66424 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sara (Verified Customer) (10-06-2024) | It works in HEK cells (human), partial colocalisation with mitochondria is seen when using Mitotracker. Very easy staining, as the antibody is already conjugated to Coralite 488. I fixed with 4% PFA, perm and stain overnight.
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