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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Prostein Monoclonal antibody (60343-1-Ig)

    WB analysis using 60343-1-Ig (same clone as 60343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using Prostein Monoclonal antibody (60343-1-Ig)

    WB analysis of DU 145 using 60343-1-Ig (same clone as 60343-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of JAR cells using Prostein Monoclonal antibody (60343-1-Ig)

    WB analysis of JAR using 60343-1-Ig (same clone as 60343-1-PBS)

    JAR cells were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Prostein Monoclonal antibody (60343-1-Ig)

    IHC staining of human prostate cancer using 60343-1-Ig (same clone as 60343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60343-1-Ig (Prostein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Prostein Monoclonal antibody (60343-1-Ig)

    IHC staining of human prostate cancer using 60343-1-Ig (same clone as 60343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60343-1-Ig (Prostein antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Prostein Monoclonal antibody (60343-1-Ig)

    IHC staining of human kidney using 60343-1-Ig (same clone as 60343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60343-1-Ig (Prostein antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Prostein Monoclonal antibody (60343-1-Ig)

    IHC staining of human kidney using 60343-1-Ig (same clone as 60343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60343-1-Ig (Prostein antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using Prostein Monoclonal antibody (60343-1-Ig)

    IF Staining of human prostate cancer using 60343-1-Ig (same clone as 60343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Prostein antibody (60343-1-Ig, Clone: 5E12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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Prostein Monoclonal antibody, PBS Only

Prostein Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60343-1-PBS
Clone No.5E12E2

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

SLC45A3, PRST, Prostate cancer-associated protein 6, PCANAP6, 5E12E2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60343-1-PBS targets Prostein in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Prostein fusion protein Ag5463 相同性解析による交差性が予測される生物種
Full Name solute carrier family 45, member 3
Calculated molecular weight 59 kDa
Observed molecular weight 55-59 kDa
GenBank accession numberBC050416
Gene Symbol Prostein
Gene ID (NCBI) 85414
RRIDAB_2881452
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96JT2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Prostein is a prostate tissue-specific protein whose expression is highly restricted to normal and cancerous prostate tissues. Anti-prostein is useful for the identification of prostate adenocarcinomas and is a good marker to identify prostatic origin in metastatic cancer.