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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Prostein Monoclonal antibody

Prostein Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

5E12E2

Cat no : 60343-1-Ig

Synonyms

SLC45A3, PRST, Prostate cancer-associated protein 6, PCANAP6, 5E12E2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Prostein Monoclonal antibody (60343-1-Ig)

    WB analysis using 60343-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using Prostein Monoclonal antibody (60343-1-Ig)

    WB analysis of DU 145 using 60343-1-Ig

    DU 145 cells were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of JAR cells using Prostein Monoclonal antibody (60343-1-Ig)

    WB analysis of JAR using 60343-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Prostein Monoclonal antibody (60343-1-Ig)

    IHC staining of human prostate cancer using 60343-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60343-1-Ig (Prostein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Prostein Monoclonal antibody (60343-1-Ig)

    IHC staining of human kidney using 60343-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60343-1-Ig (Prostein antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Prostein Monoclonal antibody (60343-1-Ig)

    IHC staining of human kidney using 60343-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60343-1-Ig (Prostein antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using Prostein Monoclonal antibody (60343-1-Ig)

    IF Staining of human prostate cancer using 60343-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Prostein antibody (60343-1-Ig, Clone: 5E12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inrat testis tissue, DU 145 cells, JAR cells, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60343-1-Ig targets Prostein in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Prostein fusion protein Ag5463 相同性解析による交差性が予測される生物種
Full Name solute carrier family 45, member 3
Calculated molecular weight 59 kDa
Observed molecular weight 55-59 kDa
GenBank accession numberBC050416
Gene symbol Prostein
Gene ID (NCBI) 85414
RRIDAB_2881452
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Prostein is a prostate tissue-specific protein whose expression is highly restricted to normal and cancerous prostate tissues. Anti-prostein is useful for the identification of prostate adenocarcinomas and is a good marker to identify prostatic origin in metastatic cancer.

Protocols

Product Specific Protocols
WB protocol for Prostein antibody 60343-1-IgDownload protocol
IHC protocol for Prostein antibody 60343-1-IgDownload protocol
IF protocol for Prostein antibody 60343-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols