Protein A Monoclonal antibody
Protein A Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
staphylococcus aureus and More (3)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1G6A6
Cat no : 66945-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | recombinant protein |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IP | See 1 publications below |
Product Information
66945-1-Ig targets Protein A in WB, IP, ELISA applications and shows reactivity with staphylococcus aureus samples.
Tested Reactivity | staphylococcus aureus |
Cited Reactivity | human, mouse, yeast |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Recombinant Protein 相同性解析による交差性が予測される生物種 |
Full Name | Protein A |
GenBank accession number | EU695225 |
Gene symbol | |
Gene ID (NCBI) | |
RRID | AB_2882269 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.
Protocols
Product Specific Protocols | |
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WB protocol for Protein A antibody 66945-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Virus Res HA2 from the Helicoverpa armigera nucleopolyhedrovirus: a WASP-related protein that activates Arp2/3-induced actin filament formation. | ||
J Biochem Title: Cloaking the ACE2 receptor with salivary cationic proteins inhibits SARS-CoV-2 entry. | ||
Hepatology Concerted synergy between viral-specific IgG and CD8 + T cells is critical for clearance of an HCV-related rodent hepacivirus | ||
Proc Natl Acad Sci U S A DNA polymerase delta governs parental histone transfer to DNA replication lagging strand |