Protein A Monoclonal antibody

Protein A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

staphylococcus aureus and More (3)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1G6A6

Cat no : 66945-1-Ig

Synonyms


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Tested Applications

Positive WB detected in recombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66945-1-Ig targets Protein A in WB, IP, ELISA applications and shows reactivity with staphylococcus aureus samples.

Tested Reactivity staphylococcus aureus
Cited Reactivityhuman, mouse, yeast
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name Protein A
GenBank accession numberEU695225
Gene symbol
Gene ID (NCBI)
RRIDAB_2882269
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.

Protocols

Product Specific Protocols
WB protocol for Protein A antibody 66945-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP

Virus Res

HA2 from the Helicoverpa armigera nucleopolyhedrovirus: a WASP-related protein that activates Arp2/3-induced actin filament formation.

Authors - Qian Wang
human

J Biochem

Title: Cloaking the ACE2 receptor with salivary cationic proteins inhibits SARS-CoV-2 entry.

Authors - Katsutoshi Yoshizato
mouseWB

Hepatology

Concerted synergy between viral-specific IgG and CD8 + T cells is critical for clearance of an HCV-related rodent hepacivirus

Authors - John Gridley
yeastWB

Proc Natl Acad Sci U S A

DNA polymerase delta governs parental histone transfer to DNA replication lagging strand

Authors - Congcong Tian