Validation Data Gallery
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Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Published Applications
| WB | See 1 publications below |
Product Information
66945-1-PBS targets Protein A in WB, Indirect ELISA applications and shows reactivity with staphylococcus aureus samples.
| Tested Reactivity | staphylococcus aureus |
| Cited Reactivity | yeast |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein 相同性解析による交差性が予測される生物種 |
| Full Name | Protein A |
| GenBank accession number | EU695225 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2882269 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Mec1-mediated Atg9 phosphorylation regulates the PAS recruitment of Atg9 vesicles upon energy stress |