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KD/KO Validated

GlnRS Polyclonal antibody

Name: GlnRS Polyclonal antibody
Brand: Proteintech
SKU: 12645-1-AP

GlnRS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12645-1-AP

Synonyms

QARS, SYQ, QARS1, Glutaminyl-tRNA synthetase, Glutamine--tRNA ligase

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 12645-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12645-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 12645-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 12645-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12645-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 12645-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12645-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of PC-3 using 12645-1-AP
IP result of anti-GlnRS (IP:12645-1-AP, 4ug; Detection:12645-1-AP 1:500) with PC-3 cells lysate 3200ug.
IHC staining of human prostate cancer using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12645-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of PC-3 using 12645-1-AP
Immunofluorescent analysis of PC-3 cells using 12645-1-AP (GlnRS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 12645-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, NIH/3T3 cells, PC-3 cells
Positive IP detected in	PC-3 cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:1500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
RIP	See 1 publications below

Product Information

12645-1-AP targets GlnRS in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GlnRS fusion protein Ag3375 相同性解析による交差性が予測される生物種
Full Name	glutaminyl-tRNA synthetase
Calculated molecular weight	775 aa, 88 kDa
Observed molecular weight	88 kDa
GenBank accession number	BC016634
Gene symbol	GlnRS
Gene ID (NCBI)	5859
RRID	AB_2098676
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GLnRS(glutaminyl-tRNA synthetase) is a class I aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The specificity of this reaction determines the fidelity of mRNA translation. At least 1 synthetase exists in the cytoplasm for each amino acid.GlnRS is present in eukaryotes but is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln)(PMID:8078941).

Protocols

Product Specific Protocols
WB protocol for GlnRS antibody 12645-1-AP	Download protocol
IHC protocol for GlnRS antibody 12645-1-AP	Download protocol
IF protocol for GlnRS antibody 12645-1-AP	Download protocol
IP protocol for GlnRS antibody 12645-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Protein Cell Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation Authors - Kai-Hui Zhang View Article
human	WB	Nat Cell Biol Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics. Authors - Maria C Passarelli View Article
human	WB	Nucleic Acids Res Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex. Authors - Haissi Cui View Article
human	WB,RIP	EMBO Rep The tRNA-like small noncoding RNA mascRNA promotes global protein translation. Authors - Xinping Lu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GlnRS Polyclonal antibody

GlnRS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 12645-1-AP

WB analysis of HepG2 using 12645-1-AP

WB analysis of PC-3 using 12645-1-AP

WB analysis of PC-3 using 12645-1-AP

WB analysis of HeLa using 12645-1-AP

WB analysis of NIH/3T3 using 12645-1-AP

WB analysis of HeLa using 12645-1-AP

IP experiment of PC-3 using 12645-1-AP

IHC staining of human prostate cancer using 12645-1-AP

IHC staining of human prostate cancer using 12645-1-AP

IF Staining of PC-3 using 12645-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation

Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.

Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.

The tRNA-like small noncoding RNA mascRNA promotes global protein translation.