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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GlnRS Polyclonal antibody

GlnRS Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12645

Synonyms

GlnRS, Glutamine tRNA ligase, glutaminyl tRNA synthetase, PRO2195, QARS

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated GlnRS Polyclonal ant (CL488-12645)

    FC experiment of HeLa using CL488-12645

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Glnrs Polyclonal Antibody (CL488-12645)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12645 targets GlnRS in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GlnRS fusion protein Ag3375 相同性解析による交差性が予測される生物種
Full Name glutaminyl-tRNA synthetase
Calculated molecular weight 775 aa, 88 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC016634
Gene symbol GlnRS
Gene ID (NCBI) 5859
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLnRS(glutaminyl-tRNA synthetase) is a class I aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The specificity of this reaction determines the fidelity of mRNA translation. At least 1 synthetase exists in the cytoplasm for each amino acid.GlnRS is present in eukaryotes but is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln)(PMID:8078941).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 GlnRS antibody CL488-12645Download protocol
Standard Protocols
Click here to view our Standard Protocols