Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
25174-1-AP targets QPRT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17680 Product name: Recombinant human QPRT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-297 aa of BC018910 Sequence: MDAEGLALLLPPVTLAALVDSWLREDCPGLNYAALVSGAGPSQAALWAKSPGVLAGQPFFDAIFTQLNCQVSWFLPEGSKLVPVARVAEVRGPAHCLLLGERVALNTLARCSGIASAAAAAVEAARGAGWTGHVAGTRKTTPGFRLVEKYGLLVGGAASHRYDLGGLVMVKDNHVVAAGGVEKAVRAARQAADFALKVEVECSSLQEAVQAAEAGADLVLLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALDFSLKLFAKEVAPVPKIH 相同性解析による交差性が予測される生物種 |
| Full Name | quinolinate phosphoribosyltransferase |
| Calculated molecular weight | 297 aa, 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC018910 |
| Gene Symbol | QPRT |
| Gene ID (NCBI) | 23475 |
| RRID | AB_2879941 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15274 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
QPRT, QAPRTase and QPRTase, is Nicotinate-nucleotide pyrophosphorylase which involved in the catabolism of quinolinic acid (QA). There're some isoforms with MW 31-33 kDa, 37 kDa and 42 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for QPRT antibody 25174-1-AP | Download protocol |
| IF protocol for QPRT antibody 25174-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Genet Copy Number Variations Analysis Identifies QPRT as a Candidate Gene Associated With Susceptibility for Solitary Functioning Kidney. | ||
J Immunol Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues | ||
J Biol Chem Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity | ||
Nat Commun Hypoxanthine phosphoribosyl transferase 1 metabolizes temozolomide to activate AMPK for driving chemoresistance of glioblastomas
| ||
Front Endocrinol (Lausanne) Unraveling the nexus of NAD+ metabolism and diabetic kidney disease: insights from murine models and human data |