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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using QPRT Polyclonal antibody (25174-1-AP)

    WB analysis using 25174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25174-1-AP (QPRT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using QPRT Polyclonal antibody (25174-1-AP)

    WB analysis of HepG2 using 25174-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25174-1-AP (QPRT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using QPRT Polyclonal antibody (25174-1-AP)

    WB analysis of L02 using 25174-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 25174-1-AP (QPRT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using QPRT Polyclonal antibody (25174-1-AP)

    IF Staining of HepG2 using 25174-1-AP

    Immunofluorescent analysis of HepG2 cells using 25174-1-AP (QPRT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

QPRT Polyclonal antibody

QPRT Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25174-1-AP
Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

QAPRTase, QPRT, QPRTase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25174-1-AP targets QPRT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen QPRT fusion protein Ag17680 相同性解析による交差性が予測される生物種
Full Name quinolinate phosphoribosyltransferase
Calculated molecular weight 297 aa, 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC018910
Gene Symbol QPRT
Gene ID (NCBI) 23475
RRIDAB_2879941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15274
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

QPRT, QAPRTase and QPRTase, is Nicotinate-nucleotide pyrophosphorylase which involved in the catabolism of quinolinic acid (QA). There're some isoforms with MW 31-33 kDa, 37 kDa and 42 kDa.

Protocols

Product Specific Protocols
WB protocol for QPRT antibody 25174-1-APDownload protocol
IF protocol for QPRT antibody 25174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Genet

Copy Number Variations Analysis Identifies QPRT as a Candidate Gene Associated With Susceptibility for Solitary Functioning Kidney.

Authors - Xiao Y Zhou
View Article
human

J Immunol

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues

Authors - Teresa W-M Fan
View Article
humanWB

J Biol Chem

Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity

Authors - Xiaohan Jin
View Article
mouseIF

Aging Cell

Deletion of enzymes for de novo NAD+ biosynthesis accelerated ovarian aging

Authors - Qingling Yang
View Article
human,mouseWB

Nat Commun

Hypoxanthine phosphoribosyl transferase 1 metabolizes temozolomide to activate AMPK for driving chemoresistance of glioblastomas

Authors - Jianxing Yin
  • KD Validated
View Article
mouseWB,IHC,IF

Front Endocrinol (Lausanne)

Unraveling the nexus of NAD+ metabolism and diabetic kidney disease: insights from murine models and human data

Authors - Sisi Yang
View Article