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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated QPRT Polyclonal antibody

QPRT Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25174

Synonyms

QPRTase, QAPRTase, Nicotinate-nucleotide pyrophosphorylase [carboxylating], EC:2.4.2.19

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated QPRT Polyclonal anti (CL488-25174)

    IF Staining of HepG2 using CL488-25174

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 QPRT antibody (CL488-25174) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated QPRT Polyclonal anti (CL488-25174)

    FC experiment of HepG2 using CL488-25174

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated QPRT Polyclonal antibody (CL488-25174)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25174 targets QPRT in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen QPRT fusion protein Ag17680 相同性解析による交差性が予測される生物種
Full Name quinolinate phosphoribosyltransferase
Calculated molecular weight 297 aa, 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC018910
Gene symbol QPRT
Gene ID (NCBI) 23475
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

QPRT, QAPRTase and QPRTase, is Nicotinate-nucleotide pyrophosphorylase which involved in the catabolism of quinolinic acid (QA). There're some isoforms with MW 31-33 kDa, 37 kDa and 42 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 QPRT antibody CL488-25174Download protocol
FC protocol for CL Plus 488 QPRT antibody CL488-25174Download protocol
Standard Protocols
Click here to view our Standard Protocols