QSOX1/Quiescin Q6 Recombinant antibody
QSOX1/Quiescin Q6 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240946B11
Cat no : 83952-3-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-1080 cells, SW 1990 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of 83952-3-RR is QSOX1/Quiescin Q6 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | QSOX1/Quiescin Q6 fusion protein Ag3396 相同性解析による交差性が予測される生物種 |
| Full Name | quiescin Q6 sulfhydryl oxidase 1 |
| Calculated molecular weight | 747 aa, 83 kDa |
| Observed molecular weight | 66-83kDa |
| GenBank accession number | BC017692 |
| Gene symbol | QSOX1 |
| Gene ID (NCBI) | 5768 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
QSOX1(Sulfhydryl oxidase 1) catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.It has two isoforms with the molecular weight of 67 kda and 83 kDa,respectively. It is detected a band of 95 Kda in HT1080 cell lines (PMID:17331072).Isoform 1 is expressed on golgi apparatus membrane and isoform 2 is secreted (PMID:10708601). The isoform1(QSOX1a) is the full length of QSOX1, approximately 78 kd; and the isoform2(QSOX1b) is the splicing form, approximately 62kd(PMID:14633699; 2496424). QSOX1 is highly expressed in tumor cell lines but is not expressed in adjacent normal cells (PMID:21989104).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for QSOX1/Quiescin Q6 antibody 83952-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
