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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RAB1B Monoclonal antibody

RAB1B Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C2G6

Cat no : CL488-67837

Synonyms

Ras-related protein Rab-1B, Ras related protein Rab 1B, EC:3.6.5.2, 3C2G6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated RAB1B Monoclonal ant (CL488-67837)

    IF Staining of MCF-7 using CL488-67837

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 RAB1B antibody (CL488-67837, Clone: 3C2G6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated RAB1B Monoclonal ant (CL488-67837)

    FC experiment of MCF-7 using CL488-67837

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAB1B (CL488-67837, Clone:3C2G6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67837 targets RAB1B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB1B fusion protein Ag12069 相同性解析による交差性が予測される生物種
Full Name RAB1B, member RAS oncogene family
Calculated molecular weight 201 aa, 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC071169
Gene symbol RAB1B
Gene ID (NCBI) 81876
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RAB1B antibody CL488-67837Download protocol
FC protocol for CL Plus 488 RAB1B antibody CL488-67837Download protocol
Standard Protocols
Click here to view our Standard Protocols