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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RAB27A Monoclonal antibody

RAB27A Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C4B8

Cat no : CL594-66058

Synonyms

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated RAB27A Monoclonal antibody (CL594-66058)

    IF Staining of HeLa using CL594-66058

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66058 (RAB27A antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66058 is RAB27A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB27A fusion protein Ag12382 相同性解析による交差性が予測される生物種
Full Name RAB27A, member RAS oncogene family
Calculated molecular weight 221 aa, 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC107680
Gene symbol RAB27A
Gene ID (NCBI) 5873
RRIDAB_2883496
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab27a is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27a is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27a is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

Protocols

Product Specific Protocols
IF protocol for CL594 RAB27A antibody CL594-66058Download protocol
Standard Protocols
Click here to view our Standard Protocols