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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RAB27B Monoclonal antibody

RAB27B Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B9G9

Cat no : CL488-66944

Synonyms

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2, 2B9G9

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated RAB27B Monoclonal an (CL488-66944)

    IF Staining of A431 using CL488-66944

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 RAB27B antibody (CL488-66944, Clone: 2B9G9 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated RAB27B Monoclonal an (CL488-66944)

    FC experiment of A431 using CL488-66944

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated RAB27B Monoclonal antibody (CL488-66944, Clone:2B9G9)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66944 targets RAB27B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAB27B fusion protein Ag18756 相同性解析による交差性が予測される生物種
Full Name RAB27B, member RAS oncogene family
Calculated molecular weight 218 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC027474
Gene symbol RAB27B
Gene ID (NCBI) 5874
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RAB27B antibody CL488-66944Download protocol
FC protocol for CL Plus 488 RAB27B antibody CL488-66944Download protocol
Standard Protocols
Click here to view our Standard Protocols