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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated RAB32 Recombinant an (CL488-82960)

    WB analysis using CL488-82960

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-82960 (RAB32 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated RAB32 Recombinant an (CL488-82960)

    FC experiment of HeLa using CL488-82960

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Rab32 Recombinant Antibody (CL488-82960, Clone:230315A9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated RAB32 Recombinant antibody

RAB32 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL488-82960
Clone No.230315A9

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra)

Synonyms

Ras-related protein Rab-32, Ras related protein Rab 32, 230315A9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82960 targets RAB32 in WB, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAB32 fusion protein Ag1450 相同性解析による交差性が予測される生物種
Full Name RAB32, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC015061
Gene Symbol RAB32
Gene ID (NCBI) 10981
RRIDAB_3673152
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13637
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 RAB32 antibody CL488-82960Download protocol
FC protocol for CL Plus 488 RAB32 antibody CL488-82960Download protocol
Standard Protocols
Click here to view our Standard Protocols