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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RAB39B Monoclonal antibody

RAB39B Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F4F7

Cat no : CL488-68014

Synonyms

Ras-related protein Rab-39B, Ras related protein Rab 39B, 2F4F7

Validation Data Gallery

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  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 488-conjugated RAB39B Monoclonal an (CL488-68014)

    FC experiment of SH-SY5Y using CL488-68014

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAB39B (CL488-68014, Clone:2F4F7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68014 targets RAB39B in FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAB39B fusion protein Ag27170 相同性解析による交差性が予測される生物種
Full Name RAB39B, member RAS oncogene family
Calculated molecular weight 213 aa, 25 kDa
Observed molecular weight25 kDa
GenBank accession numberBC009714
Gene symbol RAB39B
Gene ID (NCBI) 116442
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB39B is a member of the RAB GTPase family with a putative role in vesicle trafficking. RAB39B is predominantly expressed in neuronal cells and is localized to the endoplasmic reticulum, Golgi and trans‐Golgi recycling endosomes. Several reports suggested that RAB39B may regulate protein trafficking and the PI3K-AKT-mTOR pathway. Mutations in RAB39B are known to be associated with X-linked intellectual disability (XLID), Parkinson's disease, and autism.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 RAB39B antibody CL488-68014Download protocol
Standard Protocols
Click here to view our Standard Protocols