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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB3A Monoclonal antibody

RAB3A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B12E9

Cat no : 68052-1-Ig

Synonyms

Ras-related protein Rab-3A, Ras related protein Rab 3A, 1B12E9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB3A Monoclonal antibody (68052-1-Ig)

    WB analysis using 68052-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68052-1-Ig (RAB3A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of rat testis tissue using RAB3A Monoclonal antibody (68052-1-Ig)

    IHC staining of rat testis using 68052-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68052-1-Ig (RAB3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB3A Monoclonal antibody (68052-1-Ig)

    IHC staining of mouse brain using 68052-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68052-1-Ig (RAB3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB3A Monoclonal antibody (68052-1-Ig)

    IHC staining of mouse brain using 68052-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68052-1-Ig (RAB3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inpig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, rabbit cerebellum tissue, rat cerebellum tissue
Positive IHC detected inrat testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68052-1-Ig targets RAB3A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAB3A fusion protein Ag8148 相同性解析による交差性が予測される生物種
Full Name RAB3A, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight25 kDa
GenBank accession numberBC011782
Gene symbol RAB3A
Gene ID (NCBI) 5864
RRIDAB_2918794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab3A is a small G-protein of the Rab family, that is implicated in vesicle fusion and regulated secretion, particularly in neurotransmitter release. Rab3 subfamily small G proteins (Rab3A, Rab3B, Rab3C, and Rab3D) control the regulated exocytosis in neuronal/secretory cells. Rab3A is the most abundant Rab GTPase in brain, where it is associated with synaptic vesicles. Rab3a is believed to modulate secretion efficiency by stimulating vesicle recruitment to sites of exocytosis and/ or by recruiting regulatory molecules to the docking/ fusion machinery.

Protocols

Product Specific Protocols
WB protocol for RAB3A antibody 68052-1-IgDownload protocol
IHC protocol for RAB3A antibody 68052-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols