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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB3A Monoclonal antibody, PBS Only

RAB3A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B12E9

Cat no : 68052-1-PBS

Synonyms

Ras-related protein Rab-3A, Ras related protein Rab 3A, 1B12E9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB3A Monoclonal antibody (68052-1-Ig)

    WB analysis using 68052-1-Ig (same clone as 68052-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68052-1-Ig (RAB3A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using RAB3A Monoclonal antibody (68052-1-Ig)

    IHC staining of rat testis using 68052-1-Ig (same clone as 68052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68052-1-Ig (RAB3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB3A Monoclonal antibody (68052-1-Ig)

    IHC staining of mouse brain using 68052-1-Ig (same clone as 68052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68052-1-Ig (RAB3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RAB3A Monoclonal antibody (68052-1-Ig)

    IHC staining of mouse brain using 68052-1-Ig (same clone as 68052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68052-1-Ig (RAB3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68052-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68052-1-PBS targets RAB3A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAB3A fusion protein Ag8148 相同性解析による交差性が予測される生物種
Full Name RAB3A, member RAS oncogene family
Calculated molecular weight 25 kDa
Observed molecular weight25 kDa
GenBank accession numberBC011782
Gene symbol RAB3A
Gene ID (NCBI) 5864
RRIDAB_2918794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Rab3A is a small G-protein of the Rab family, that is implicated in vesicle fusion and regulated secretion, particularly in neurotransmitter release. Rab3 subfamily small G proteins (Rab3A, Rab3B, Rab3C, and Rab3D) control the regulated exocytosis in neuronal/secretory cells. Rab3A is the most abundant Rab GTPase in brain, where it is associated with synaptic vesicles. Rab3a is believed to modulate secretion efficiency by stimulating vesicle recruitment to sites of exocytosis and/ or by recruiting regulatory molecules to the docking/ fusion machinery.