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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RAB3GAP1 Monoclonal antibody

RAB3GAP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4A3A1

Cat no : CL488-66688

Synonyms

RAB3 GTPase-activating protein 130 kDa subunit, Rab3 GAP p130, Rab3 GAP, P130, KIAA0066

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated RAB3GAP1 Monoclonal (CL488-66688)

    IF Staining of HEK-293 using CL488-66688

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 RAB3GAP1 antibody (CL488-66688, Clone: 4A3A1 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated RAB3GAP1 Monoclonal (CL488-66688)

    FC experiment of HEK-293 using CL488-66688

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAB3GAP1 (CL488-66688, Clone:4A3A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66688 targets RAB3GAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB3GAP1 fusion protein Ag17710 相同性解析による交差性が予測される生物種
Full Name RAB3 GTPase activating protein subunit 1 (catalytic)
Calculated molecular weight 981 aa, 111 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC146809
Gene symbol RAB3GAP1
Gene ID (NCBI) 22930
RRIDAB_2919358
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RAB3GAP1 antibody CL488-66688Download protocol
FC protocol for CL Plus 488 RAB3GAP1 antibody CL488-66688Download protocol
Standard Protocols
Click here to view our Standard Protocols