Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, fetal human brain tissue, pig brain tissue |
| Positive IHC detected in | human liver cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
66339-1-Ig targets RAB5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RAB5A fusion protein Ag16781 相同性解析による交差性が予測される生物種 |
| Full Name | RAB5A, member RAS oncogene family |
| Calculated molecular weight | 215 aa, 24 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC001267 |
| Gene Symbol | RAB5A |
| Gene ID (NCBI) | 5868 |
| RRID | AB_2881719 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20339 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB5A antibody 66339-1-Ig | Download protocol |
| IHC protocol for RAB5A antibody 66339-1-Ig | Download protocol |
| IF protocol for RAB5A antibody 66339-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis. | ||
Clin Ther Differential Expression of Rab5 and Rab7 Small GTPase Proteins in Placental Tissues From Pregnancies Affected by Maternal Coronavirus Disease 2019. | ||
J Exp Clin Cancer Res TFEB-driven autophagy potentiates TGF-β induced migration in pancreatic cancer cells. | ||
Commun Biol Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium. | ||
PLoS Pathog ALIX and TSG101 are essential for cellular entry and replication of two porcine alphacoronaviruses | ||
Cell Rep Pten regulates endocytic trafficking of cell adhesion and Wnt signaling molecules to pattern the retina |