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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RAB5A Monoclonal antibody (66339-1-Ig)

    WB analysis of HEK-293 using 66339-1-Ig (same clone as 66339-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using RAB5A Monoclonal antibody (66339-1-Ig)

    WB analysis of fetal human brain using 66339-1-Ig (same clone as 66339-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using RAB5A Monoclonal antibody (66339-1-Ig)

    WB analysis of pig brain using 66339-1-Ig (same clone as 66339-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RAB5A Monoclonal antibody (66339-1-Ig)

    IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RAB5A Monoclonal antibody (66339-1-Ig)

    IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using RAB5A Monoclonal antibody (66339-1-Ig)

    IHC staining of human pancreas using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using RAB5A Monoclonal antibody (66339-1-Ig)

    IHC staining of human pancreas using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAB5A Monoclonal antibody (66339-1-Ig)

    IF Staining of HepG2 using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB5A Monoclonal antibody (66339-1-Ig)

    IF Staining of HeLa using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RAB5A Monoclonal antibody (66339-1-Ig)

    IF Staining of HepG2 using 66339-1-Ig (same clone as 66339-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66339-1-Ig(RAB5A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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RAB5A Monoclonal antibody, PBS Only

RAB5A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66339-1-PBS
Clone No.1B6A5

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66339-1-PBS targets RAB5A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAB5A fusion protein Ag16781 相同性解析による交差性が予測される生物種
Full Name RAB5A, member RAS oncogene family
Calculated molecular weight 215 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC001267
Gene Symbol RAB5A
Gene ID (NCBI) 5868
RRIDAB_2881719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20339
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.