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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB5A Recombinant antibody (83949-4-RR)

    WB analysis using 83949-4-RR (same clone as 83949-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83949-4-RR (RAB5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83949-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83949-4-RR

    Affinity and Kinetic Characterization of 83949-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83949-4-RR against Human RAB5A were performed. The affinity constant is 26.7 nM.

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RAB5A Recombinant antibody, PBS Only

RAB5A Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 83949-4-PBS
Clone No.241041D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

RAB5, Ras-related protein Rab-5A, Ras related protein Rab 5A, EC:3.6.5.2, 241041D7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83949-4-PBS targets RAB5A in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAB5A fusion protein Ag2549 相同性解析による交差性が予測される生物種
Full Name RAB5A, member RAS oncogene family
Calculated molecular weight 215 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC001267
Gene Symbol RAB5A
Gene ID (NCBI) 5868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP20339
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.