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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB5A Recombinant antibody (83949-4-RR)

    WB analysis using 83949-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83949-4-RR (RAB5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83949-4-RR

    Affinity and Kinetic Characterization of 83949-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83949-4-RR against Human RAB5A were performed. The affinity constant is 26.7 nM.

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RAB5A Recombinant antibody

RAB5A Recombinant Antibody for WB, ELISA
Cat No. 83949-4-RR
Clone No.241041D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RAB5, Ras-related protein Rab-5A, Ras related protein Rab 5A, EC:3.6.5.2, 241041D7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tisue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83949-4-RR targets RAB5A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAB5A fusion protein Ag2549 相同性解析による交差性が予測される生物種
Full Name RAB5A, member RAS oncogene family
Calculated molecular weight 215 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC001267
Gene Symbol RAB5A
Gene ID (NCBI) 5868
RRIDAB_3671528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP20339
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.

Protocols

Product Specific Protocols
WB protocol for RAB5A antibody 83949-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols