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RAB5B Polyclonal antibody

Name: RAB5B Polyclonal antibody
Brand: Proteintech
SKU: 27403-1-AP
Rating: 4.00 (1 reviews)

RAB5B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 27403-1-AP

Synonyms

Ras-related protein Rab-5B, Ras related protein Rab 5B, EC:3.6.5.2

Validation Data Gallery

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AllWBIHCIF/ICC

WB analysis of HeLa using 27403-1-AP
WB result of RAB5B antibody (27403-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB5B transfected HeLa cells.
WB analysis of mouse lung using 27403-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 27403-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 27403-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 27403-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 27403-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 27403-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 27403-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27403-1-AP (RAB5B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 27403-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27403-1-AP (RAB5B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
IF Staining of NIH/3T3 using 27403-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 27403-1-AP (RAB5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 27403-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse lung tissue, Jurkat cells, HeLa cells, HepG2 cells, NIH/3T3 cells, HEK-293 cells
Positive IHC detected in	human stomach tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 1 publications below
FC	See 1 publications below

Product Information

27403-1-AP targets RAB5B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAB5B fusion protein Ag26584 相同性解析による交差性が予測される生物種
Full Name	RAB5B, member RAS oncogene family
Calculated molecular weight	215 aa, 24 kDa
Observed molecular weight	27 kDa
GenBank accession number	BC032740
Gene symbol	RAB5B
Gene ID (NCBI)	5869
RRID	AB_2880862
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAB5B (Ras-related protein Rab-5B) belongs to the Rab family. Rab5 is specifically localized to the early compartment and regulates endocytosis (PMID:1516130, PMID:9087439). There are three isoforms of Rab5 namely, Rab5a, Rab5b, and Rab5c present in EE of mammalian cells (PMID:7789520). Recently, we have shown that Rab5a regulates fluid-phase endocytosis whereas receptor-mediated endocytosis is regulated by Rab5b in Leishmania (PMID:27226564).

Protocols

Product Specific Protocols
WB protocol for RAB5B antibody 27403-1-AP	Download protocol
IHC protocol for RAB5B antibody 27403-1-AP	Download protocol
IF protocol for RAB5B antibody 27403-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	bioRxiv The Sde Phosphoribosyl-Linked Ubiquitin Transferases protect the Legionella pneumophila vacuole from degradation by the host Authors - Seongok Kim KD Validated View Article
mouse	WB	Proc Natl Acad Sci U S A The Sde phosphoribosyl-linked ubiquitin transferases protect the Legionella pneumophila vacuole from degradation by the host Authors - Seongok Kim View Article
human,mouse	IHC,WB,FC	Mol Hum Reprod Hsa_circRNA_BECN1 acts as a ceRNA to promote polycystic ovary syndrome progression by sponging the miR-619-5p/Rab5b axis Authors - Hairui Fan View Article
mouse	WB	Development A crucial role for Arf6 in the response of commissural axons to Slit. Authors - Mariko Kinoshita-Kawada View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Xin (Verified Customer) (04-16-2022)

It is ok to detect endogenous Rab5B, although the signal is a little weak.

Applications: Western Blot
Primary Antibody Dilution: 1:1000
Cell Tissue Type: mouse cortex lysate

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB5B Polyclonal antibody

RAB5B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HeLa using 27403-1-AP

WB analysis of mouse lung using 27403-1-AP

WB analysis of Jurkat using 27403-1-AP

WB analysis of HeLa using 27403-1-AP

WB analysis of HepG2 using 27403-1-AP

WB analysis of NIH/3T3 using 27403-1-AP

WB analysis of HEK-293 using 27403-1-AP

IHC staining of human stomach using 27403-1-AP

IHC staining of human colon using 27403-1-AP

IF Staining of NIH/3T3 using 27403-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The Sde Phosphoribosyl-Linked Ubiquitin Transferases protect the Legionella pneumophila vacuole from degradation by the host

The Sde phosphoribosyl-linked ubiquitin transferases protect the Legionella pneumophila vacuole from degradation by the host

Hsa_circRNA_BECN1 acts as a ceRNA to promote polycystic ovary syndrome progression by sponging the miR-619-5p/Rab5b axis

A crucial role for Arf6 in the response of commissural axons to Slit.

Reviews