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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RAB5B Polyclonal antibody

RAB5B Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-27403

Synonyms

Ras-related protein Rab-5B, Ras related protein Rab 5B, EC:3.6.5.2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite® Plus 488-conjugated RAB5B Polyclonal ant (CL488-27403)

    IF Staining of NIH/3T3 using CL488-27403

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 RAB5B antibody (CL488-27403) at dilution of 1:200.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite® Plus 488-conjugated RAB5B Polyclonal ant (CL488-27403)

    FC experiment of NIH/3T3 using CL488-27403

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Rab5b Polyclonal Antibody (CL488-27403)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-27403 targets RAB5B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB5B fusion protein Ag26584 相同性解析による交差性が予測される生物種
Full Name RAB5B, member RAS oncogene family
Calculated molecular weight 215 aa, 24 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC032740
Gene symbol RAB5B
Gene ID (NCBI) 5869
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB5B (Ras-related protein Rab-5B) belongs to the Rab family. Rab5 is specifically localized to the early compartment and regulates endocytosis (PMID:1516130, PMID:9087439). There are three isoforms of Rab5 namely, Rab5a, Rab5b, and Rab5c present in EE of mammalian cells (PMID:7789520). Recently, we have shown that Rab5a regulates fluid-phase endocytosis whereas receptor-mediated endocytosis is regulated by Rab5b in Leishmania (PMID:27226564).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RAB5B antibody CL488-27403Download protocol
FC protocol for CL Plus 488 RAB5B antibody CL488-27403Download protocol
Standard Protocols
Click here to view our Standard Protocols