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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB7A Recombinant antibody (84741-1-RR)

    WB analysis using 84741-1-RR (same clone as 84741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84741-1-RR (RAB7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RAB7A Recombinant antibody (84741-1-RR)

    IF Staining of A431 using 84741-1-RR (same clone as 84741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB7A Recombinant antibody (84741-1-RR)

    IF Staining of HeLa using 84741-1-RR (same clone as 84741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using RAB7A Recombinant antibody (84741-1-RR)

    FC experiment of A431 using 84741-1-RR (same clone as 84741-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using RAB7A Recombinant antibody (84741-1-RR)

    FC experiment of K-562 using 84741-1-RR (same clone as 84741-1-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84741-1-PBS in a different storage buffer formulation.

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RAB7A Recombinant antibody, PBS Only

RAB7A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84741-1-PBS
Clone No.241948B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

RAB7, Ras-related protein Rab-7a, Ras related protein Rab 7a, EC:3.6.5.2, 241948B4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84741-1-PBS targets RAB7A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name RAB7A, member RAS oncogene family
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberNM_004637
Gene Symbol RAB7A
Gene ID (NCBI) 7879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP51149
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.