• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RAB7A Recombinant antibody (84741-1-RR)

    WB analysis using 84741-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84741-1-RR (RAB7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RAB7A Recombinant antibody (84741-1-RR)

    IF Staining of A431 using 84741-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB7A Recombinant antibody (84741-1-RR)

    IF Staining of HeLa using 84741-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB7A antibody (84741-1-RR, Clone: 241948B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using RAB7A Recombinant antibody (84741-1-RR)

    FC experiment of A431 using 84741-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of K-562 cells using RAB7A Recombinant antibody (84741-1-RR)

    FC experiment of K-562 using 84741-1-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB7A Recombinant antibody (84741-1-RR, Clone:241948B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

RAB7A Recombinant antibody

RAB7A Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84741-1-RR
Clone No.241948B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

RAB7, Ras-related protein Rab-7a, Ras related protein Rab 7a, EC:3.6.5.2, 241948B4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, L-929 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inA431 cells, HeLa cells
Positive FC (Intra) detected inK-562 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84741-1-RR targets RAB7A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name RAB7A, member RAS oncogene family
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberNM_004637
Gene Symbol RAB7A
Gene ID (NCBI) 7879
RRIDAB_3672126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP51149
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.

Protocols

Product Specific Protocols
WB protocol for RAB7A antibody 84741-1-RRDownload protocol
IF protocol for RAB7A antibody 84741-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols