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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RABEP1 Monoclonal antibody

RABEP1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E7E11

Cat no : CL488-68285

Synonyms

RAB5EP, Rabaptin 4, Rabaptin 5, Rabaptin 5alpha, RABEP1, RABPT5, RABPT5A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated RABEP1 Monoclonal an (CL488-68285)

    IF Staining of HeLa using CL488-68285

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 RABEP1 antibody (CL488-68285, Clone: 1E7E11 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68285 targets RABEP1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RABEP1 fusion protein Ag6023 相同性解析による交差性が予測される生物種
Full Name rabaptin, RAB GTPase binding effector protein 1
Calculated molecular weight 99 kDa
Observed molecular weight100-105 kDa
GenBank accession numberBC041700
Gene symbol RABEP1
Gene ID (NCBI) 9135
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab GTPase-binding effector protein 1 is an enzyme that in humans is encoded by the RABEP1 gene. It belongs to rabaptin protein family. RABEP1 act as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. (PMID: 22841712)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RABEP1 antibody CL488-68285Download protocol
Standard Protocols
Click here to view our Standard Protocols