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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RABEPK/p40 Polyclonal antibody

RABEPK/p40 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15105-1-AP

Synonyms

p40, RABEPK, RAB9P40, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RABEPK/p40 Polyclonal antibody (15105-1-AP)

    WB analysis of HepG2 using 15105-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RABEPK/p40 Polyclonal antibody (15105-1-AP)

    WB analysis using 15105-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RABEPK/p40 Polyclonal antibody (15105-1-AP)

    WB analysis of HeLa using 15105-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RABEPK/p40 Polyclonal antibody (15105-1-AP)

    WB analysis of K-562 using 15105-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RABEPK/p40 Polyclonal antibody (15105-1-AP)

    IP experiment of HeLa using 15105-1-AP

    IP result of anti-RABEPK/p40 (IP:15105-1-AP, 4ug; Detection:15105-1-AP 1:1000) with HeLa cells lysate 2080 ug.

  • Immunohistochemistry (IHC) staining of human lung squamous cell carcinoma tissue using RABEPK/p40 Polyclonal antibody (15105-1-AP)

    IHC staining of human lung squamous cell carcinoma using 15105-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RABEPK/p40 Polyclonal antibody (15105-1-AP)

    IF Staining of HeLa using 15105-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEPK/p40 antibody (15105-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, K-562 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung squamous cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15105-1-AP targets RABEPK/p40 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABEPK/p40 fusion protein Ag2593 相同性解析による交差性が予測される生物種
Full Name Rab9 effector protein with kelch motifs
Calculated molecular weight 41 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC065725
Gene symbol RABEPK/p40
Gene ID (NCBI) 10244
RRIDAB_1557623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

Protocols

Product Specific Protocols
WB protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
IHC protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
IF protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
IP protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols