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Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated RABEPK/p40 Polyclonal antibody

RABEPK/p40 Polyclonal Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-15105

Synonyms

p40, RABEPK, RAB9P40, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite®555-conjugated RABEPK/p40 Polyclonal anti (CL555-15105)

    WB analysis using CL555-15105

    Various lysates were subjected to SDS PAGE followed by western blot with CL555-15105 (RABEPK/p40 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated RABEPK/p40 Polyclonal anti (CL555-15105)

    FC experiment of HeLa using CL555-15105

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human RABEPK/p40 (CL555-15105) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-15105 targets RABEPK/p40 in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABEPK/p40 fusion protein Ag2593 相同性解析による交差性が予測される生物種
Full Name Rab9 effector protein with kelch motifs
Calculated molecular weight 41 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC065725
Gene symbol RABEPK/p40
Gene ID (NCBI) 10244
RRIDAB_2919623
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

Protocols

Product Specific Protocols
WB protocol for CL555 RABEPK/p40 antibody CL555-15105Download protocol
FC protocol for CL555 RABEPK/p40 antibody CL555-15105Download protocol
Standard Protocols
Click here to view our Standard Protocols