• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

RACGAP1 Monoclonal antibody

RACGAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6E10A2

Cat no : 66056-1-Ig

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using RACGAP1 Monoclonal antibody (66056-1-Ig)

    WB analysis of HEK-293 using 66056-1-Ig

    HEK-293, Jurkat, ROS1728, PC-12, Raw264.7, NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK293 cells using RACGAP1 Monoclonal antibody (66056-1-Ig)

    WB analysis of HEK293 cells using 66056-1-Ig

    WB result of RACGAP1 antibody (66056-1-Ig, 1:3000) with si-control and si-RACGAP1 transfected HEK293 cells.

  • Western Blot (WB) analysis of human brain tissue using RACGAP1 Monoclonal antibody (66056-1-Ig)

    WB analysis of human brain using 66056-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RACGAP1 Monoclonal antibody (66056-1-Ig)

    WB analysis of HeLa using 66056-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RACGAP1 Monoclonal antibody (66056-1-Ig)

    WB analysis of HEK-293 using 66056-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RACGAP1 Monoclonal antibody (66056-1-Ig)

    IHC staining of human colon cancer using 66056-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RACGAP1 Monoclonal antibody (66056-1-Ig)

    IHC staining of human colon cancer using 66056-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RACGAP1 Monoclonal antibody (66056-1-Ig)

    IF Staining of HeLa using 66056-1-Ig

    Immunofluorescent analysis of HeLa cells, using RACGAP1 antibody 66056-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inHEK-293 cells, HEK293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66056-1-Ig is RACGAP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RACGAP1 fusion protein Ag6944 相同性解析による交差性が予測される生物種
Full Name Rac GTPase activating protein 1
Calculated molecular weight 632 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC032754
Gene symbol RACGAP1
Gene ID (NCBI) 29127
RRIDAB_11043178
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

Protocols

Product Specific Protocols
WB protocol for RACGAP1 antibody 66056-1-IgDownload protocol
IHC protocol for RACGAP1 antibody 66056-1-IgDownload protocol
IF protocol for RACGAP1 antibody 66056-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols