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  • Featured Product
  • KD/KO Validated

RACGAP1 Monoclonal antibody, PBS Only

RACGAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6E10A2

Cat no : 66056-1-PBS

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RACGAP1 Monoclonal antibody (66056-1-Ig)

    WB analysis of HEK-293 using 66056-1-Ig (same clone as 66056-1-PBS)

    HEK-293, Jurkat, ROS1728, PC-12, Raw264.7, NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK293 cells using RACGAP1 Monoclonal antibody (66056-1-Ig)

    WB analysis of HEK293 cells using 66056-1-Ig (same clone as 66056-1-PBS)

    WB result of RACGAP1 antibody (66056-1-Ig, 1:3000) with si-control and si-RACGAP1 transfected HEK293 cells. This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RACGAP1 Monoclonal antibody (66056-1-Ig)

    WB analysis of human brain using 66056-1-Ig (same clone as 66056-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RACGAP1 Monoclonal antibody (66056-1-Ig)

    WB analysis of HeLa using 66056-1-Ig (same clone as 66056-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RACGAP1 Monoclonal antibody (66056-1-Ig)

    WB analysis of HEK-293 using 66056-1-Ig (same clone as 66056-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RACGAP1 Monoclonal antibody (66056-1-Ig)

    IHC staining of human colon cancer using 66056-1-Ig (same clone as 66056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RACGAP1 Monoclonal antibody (66056-1-Ig)

    IHC staining of human colon cancer using 66056-1-Ig (same clone as 66056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RACGAP1 Monoclonal antibody (66056-1-Ig)

    IF Staining of HeLa using 66056-1-Ig (same clone as 66056-1-PBS)

    Immunofluorescent analysis of HeLa cells, using RACGAP1 antibody 66056-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66056-1-PBS targets RACGAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RACGAP1 fusion protein Ag6944 相同性解析による交差性が予測される生物種
Full Name Rac GTPase activating protein 1
Calculated molecular weight 632 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC032754
Gene symbol RACGAP1
Gene ID (NCBI) 29127
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.