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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD1 Monoclonal antibody (68637-1-Ig)

    WB analysis using 68637-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68637-1-Ig (RAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RAD1 Monoclonal antibody (68637-1-Ig)

    IP experiment of HeLa using 68637-1-Ig

    IP result of anti-RAD1 (IP:68637-1-Ig, 4ug; Detection:68637-1-Ig 1:1000) with HeLa cells lysate 1320 ug.

  • Flow cytometry (FC) experiment of HeLa cells using RAD1 Monoclonal antibody (68637-1-Ig)

    FC experiment of HeLa using 68637-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAD1 Monoclonal antibody (68637-1-Ig, Clone:1A11E2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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RAD1 Monoclonal antibody

RAD1 Monoclonal Antibody for WB, FC (Intra), IP, ELISA
Cat No. 68637-1-Ig
Clone No.1A11E2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Synonyms

REC1, Rad1-like DNA damage checkpoint protein, HRAD1, 1A11E2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMKN-45 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, Raji cells, Daudi cells, K-562 cells
Positive IP detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68637-1-Ig targets RAD1 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAD1 fusion protein Ag33257 相同性解析による交差性が予測される生物種
Full Name RAD1 homolog (S. pombe)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC006837
Gene Symbol RAD1
Gene ID (NCBI) 5810
RRIDAB_3085326
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60671
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for RAD1 antibody 68637-1-IgDownload protocol
IP protocol for RAD1 antibody 68637-1-IgDownload protocol
FC protocol for RAD1 antibody 68637-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols