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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD1 Monoclonal antibody (68637-1-Ig)

    WB analysis using 68637-1-Ig (same clone as 68637-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68637-1-Ig (RAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RAD1 Monoclonal antibody (68637-1-Ig)

    IP experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

    IP result of anti-RAD1 (IP:68637-1-Ig, 4ug; Detection:68637-1-Ig 1:1000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RAD1 Monoclonal antibody (68637-1-Ig)

    FC experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAD1 Monoclonal antibody (68637-1-Ig, Clone:1A11E2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

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RAD1 Monoclonal antibody, PBS Only

RAD1 Monoclonal Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 68637-1-PBS
Clone No.1A11E2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra), IP, Indirect ELISA

Synonyms

REC1, Rad1-like DNA damage checkpoint protein, HRAD1, 1A11E2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68637-1-PBS targets RAD1 in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAD1 fusion protein Ag33257 相同性解析による交差性が予測される生物種
Full Name RAD1 homolog (S. pombe)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC006837
Gene Symbol RAD1
Gene ID (NCBI) 5810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60671
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.