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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAD17 Monoclonal antibody

RAD17 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F12D8

Cat no : 68539-1-Ig

Synonyms

CCYC, FLJ41520, HRAD17, R24L, RAD17, RAD17 homolog (S. pombe), RAD17SP, RAD24, RF C/activator 1 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD17 Monoclonal antibody (68539-1-Ig)

    WB analysis using 68539-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68539-1-Ig (RAD17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68539-1-Ig targets RAD17 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAD17 fusion protein Ag4348 相同性解析による交差性が予測される生物種
Full Name RAD17 homolog (S. pombe)
Calculated molecular weight 681 aa, 71 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC032304
Gene symbol RAD17
Gene ID (NCBI) 5884
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rad17, a component of the checkpoint clamp loader complex (RAD17/RFC), was originally initially identified as a DNA damage checkpoint protein. Rad17 participates in cell cycle control and DNA damage repair. Rad17 phosphorylation by ATR (ataxia telangiectasia mutated and Rad3-related) kinase is indispensable for the recruitment of Claspin and the activation of Chk1 in response to genotoxic stress.

Protocols

Product Specific Protocols
WB protocol for RAD17 antibody 68539-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols