• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAE1 Recombinant antibody, PBS Only (Capture/Detector)

RAE1 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240861E12

Cat no : 83712-1-PBS

Synonyms

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RAE1 Recombinant antibody (83712-1-RR)

    WB analysis using 83712-1-RR (same clone as 83712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83712-1-RR (RAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using RAE1 Recombinant antibody (83712-1-RR)

    FC experiment of HepG2 using 83712-1-RR (same clone as 83712-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAE1 Recombinant antibody (83712-1-RR, Clone:240861E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00683-1

    Cytometric bead array standard curve of MP00683-1

    Cytometric bead array standard curve of MP00683-1, RAE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83712-3-PBS. Detection antibody: 83712-1-PBS. Standard: Ag12102. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00683-3

    Cytometric bead array standard curve of MP00683-3

    Cytometric bead array standard curve of MP00683-3, RAE1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83712-1-PBS. Detection antibody: 83712-2-PBS. Standard: Ag12102. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83712-1-RR

    Affinity and Kinetic Characterization of 83712-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83712-1-RR against Human RAE1 were performed. The affinity constant is 1.36 nM.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83712-1-PBS targets RAE1 as part of a matched antibody pair:

MP00683-1: 83712-3-PBS capture and 83712-1-PBS detection (validated in Cytometric bead array)

MP00683-3: 83712-1-PBS capture and 83712-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAE1 fusion protein Ag12102 相同性解析による交差性が予測される生物種
Full Name RAE1 RNA export 1 homolog (S. pombe)
Calculated molecular weight 368 aa, 41 kDa
Observed molecular weight41 kDa
GenBank accession numberBC103754
Gene symbol RAE1
Gene ID (NCBI) 8480
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)