RAE1 Recombinant antibody
RAE1 Recombinant Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240861E12
Cat no : 83712-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, MCF-7 cells, HeLa cells, HEK-293 cells, BxPC-3 cells, mouse brain tissue, mouse thymus tissue |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83712-1-RR is RAE1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | RAE1 fusion protein Ag12102 相同性解析による交差性が予測される生物種 |
Full Name | RAE1 RNA export 1 homolog (S. pombe) |
Calculated molecular weight | 368 aa, 41 kDa |
Observed molecular weight | 41 kDa |
GenBank accession number | BC103754 |
Gene symbol | RAE1 |
Gene ID (NCBI) | 8480 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)
Protocols
Product Specific Protocols | |
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WB protocol for RAE1 antibody 83712-1-RR | Download protocol |
FC protocol for RAE1 antibody 83712-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |