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RALA Monoclonal antibody

RALA Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, FC (Intra), ELISA, IF

Conjugate

Unconjugated

CloneNo.

1H9H5

Cat no : 67093-1-Ig

Synonyms

Ras-related protein Ral-A, Ras related protein Ral A, RAL, EC:3.6.5.2, 1H9H5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RALA Monoclonal antibody (67093-1-Ig)

    WB analysis using 67093-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67093-1-Ig (RALA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RALA Monoclonal antibody (67093-1-Ig)

    WB analysis of HeLa using 67093-1-Ig

    WB result of RALA antibody (67093-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RALA transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using RALA Monoclonal antibody (67093-1-Ig)

    WB analysis using 67093-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67093-1-Ig (RALA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RALA Monoclonal antibody (67093-1-Ig)

    IHC staining of human breast cancer using 67093-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67093-1-Ig (RALA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RALA Monoclonal antibody (67093-1-Ig)

    IHC staining of human breast cancer using 67093-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67093-1-Ig (RALA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of MCF-7 cells using RALA Monoclonal antibody (67093-1-Ig)

    FC experiment of MCF-7 using 67093-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RALA (67093-1-Ig, Clone:1H9H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, pig brain tissue, MCF-7 cells, HepG2 cells, Jurkat cells, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

67093-1-Ig targets RALA in WB, IHC, FC (Intra), ELISA, IF applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RALA fusion protein Ag5329 相同性解析による交差性が予測される生物種
Full Name v-ral simian leukemia viral oncogene homolog A (ras related)
Calculated molecular weight 206 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC039858
Gene symbol RALA
Gene ID (NCBI) 5898
RRIDAB_2882398
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RALA antibody 67093-1-IgDownload protocol
IHC protocol for RALA antibody 67093-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cancers (Basel)

Controlled Plasma Membrane Delivery of FGFR1 and Modulation of Signaling by a Novel Regulated Anterograde RTK Transport Pathway

Authors - Claire Leist Hinsch
View Article