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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RALA Monoclonal antibody

RALA Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H9H5

Cat no : CL594-67093

Synonyms

Ras-related protein Ral-A, Ras related protein Ral A, RAL, EC:3.6.5.2, 1H9H5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated RALA Monoclonal antibody (CL594-67093)

    FC experiment of MCF-7 using CL594-67093

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human RALA (CL594-67093, Clone:1H9H5) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67093 targets RALA in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RALA fusion protein Ag5329 相同性解析による交差性が予測される生物種
Full Name v-ral simian leukemia viral oncogene homolog A (ras related)
Calculated molecular weight 206 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC039858
Gene symbol RALA
Gene ID (NCBI) 5898
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL594 RALA antibody CL594-67093Download protocol
Standard Protocols
Click here to view our Standard Protocols