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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RALB Monoclonal antibody (67094-1-Ig)

    WB analysis using 67094-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RALB Monoclonal antibody (67094-1-Ig)

    WB analysis using 67094-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using RALB Monoclonal antibody (67094-1-Ig)

    WB analysis of rat brain using 67094-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RALB Monoclonal antibody (67094-1-Ig)

    WB analysis of mouse brain using 67094-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using RALB Monoclonal antibody (67094-1-Ig)

    IHC staining of mouse bladder using 67094-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using RALB Monoclonal antibody (67094-1-Ig)

    IHC staining of mouse placenta using 67094-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using RALB Monoclonal antibody (67094-1-Ig)

    IHC staining of mouse liver using 67094-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using RALB Monoclonal antibody (67094-1-Ig)

    IHC staining of mouse lung using 67094-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RALB Monoclonal antibody (67094-1-Ig)

    IHC staining of human lung cancer using 67094-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using RALB Monoclonal antibody (67094-1-Ig)

    IF Staining of human lung cancer using 67094-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using RALB antibody (67094-1-Ig, Clone: 1D11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RALB Monoclonal antibody

RALB Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67094-1-Ig
Clone No.1D11C11
Publications(2)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

RALB, Ras related protein Ral B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, rat brain tissue, mouse brain tissue, MCF-7 cells, HepG2 cells, Jurkat cells, Pig brain cells
Positive IHC detected inmouse bladder tissue, human lung cancer tissue, mouse liver tissue, mouse lung tissue, mouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67094-1-Ig targets RALB in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

Tested Reactivity Human, rat, pig, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Affinity
KD=1.82 x 10-10M
KOff=2.54 x 10-6M
KOn=1.40 x 104M
Immunogen RALB fusion protein Ag28600 相同性解析による交差性が予測される生物種
Full Name v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
Calculated molecular weight 206 aa, 23 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC018163
Gene Symbol RALB
Gene ID (NCBI) 5899
RRIDAB_2882399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11234
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RALB antibody 67094-1-IgDownload protocol
IHC protocol for RALB antibody 67094-1-IgDownload protocol
IF protocol for RALB antibody 67094-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Hum Pathol

Papillary renal neoplasm with reverse polarity: a clinicopathologic study of 43 cases with a focus on the expression of KRAS signaling pathway downstream effectors

Authors - Bohyun Kim
View Article
humanIF

Cancers (Basel)

Controlled Plasma Membrane Delivery of FGFR1 and Modulation of Signaling by a Novel Regulated Anterograde RTK Transport Pathway

Authors - Claire Leist Hinsch
View Article