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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RALB Monoclonal antibody, PBS Only (Detector)

RALB Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig, mouse

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11C11

Cat no : 67094-1-PBS

Synonyms

RALB, Ras related protein Ral B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RALB Monoclonal antibody (67094-1-Ig)

    WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RALB Monoclonal antibody (67094-1-Ig)

    WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using RALB Monoclonal antibody (67094-1-Ig)

    WB analysis of rat brain using 67094-1-Ig (same clone as 67094-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using RALB Monoclonal antibody (67094-1-Ig)

    WB analysis of mouse brain using 67094-1-Ig (same clone as 67094-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using RALB Monoclonal antibody (67094-1-Ig)

    IHC staining of mouse placenta using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using RALB Monoclonal antibody (67094-1-Ig)

    IHC staining of mouse bladder using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using RALB Monoclonal antibody (67094-1-Ig)

    IHC staining of mouse liver using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using RALB Monoclonal antibody (67094-1-Ig)

    IHC staining of mouse lung using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RALB Monoclonal antibody (67094-1-Ig)

    IHC staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using RALB Monoclonal antibody (67094-1-Ig)

    IF Staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using RALB antibody (67094-1-Ig, Clone: 1D11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50129-1

    Cytometric bead array standard curve of MP50129-1

    Cytometric bead array standard curve of MP50129-1, RALB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67094-2-PBS. Detection antibody: 67094-1-PBS. Standard:Ag28600. Range: 6.25-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67094-1-PBS targets RALB as part of a matched antibody pair:

MP50129-1: 67094-2-PBS capture and 67094-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human, rat, pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Affinity
KD=1.82 x 10-10M
KOff=2.54 x 10-6M
KOn=1.40 x 104M
Immunogen RALB fusion protein Ag28600 相同性解析による交差性が予測される生物種
Full Name v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
Calculated molecular weight 206 aa, 23 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC018163
Gene symbol RALB
Gene ID (NCBI) 5899
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.