Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
67146-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-67146 targets RANGAP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RANGAP1 fusion protein Ag26136 相同性解析による交差性が予測される生物種 |
| Full Name | Ran GTPase activating protein 1 |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 64 kDa, 82 kDa |
| GenBank accession number | BC014044 |
| Gene Symbol | RANGAP1 |
| Gene ID (NCBI) | 5905 |
| RRID | AB_3084753 |
| Conjugate | CoraLite® Plus 594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 594 nm / 615 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P46060 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 594 RANGAP1 antibody CL594-67146 | Download protocol |
| FC protocol for CL Plus 594 RANGAP1 antibody CL594-67146 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |