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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 594-conjugated RANGAP1 Monoclonal antibody

RANGAP1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

1F3A4

Cat no : CL594-67146

Synonyms

SD, Ran GTPase-activating protein 1, KIAA1835, 1F3A4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 594-conjugated RANGAP1 Monoclonal a (CL594-67146)

    IF Staining of HepG2 using CL594-67146

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 594 RANGAP1 antibody (CL594-67146, Clone: 1F3A4 ) at dilution of 1:1000.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 594-conjugated RANGAP1 Monoclonal a (CL594-67146)

    FC experiment of HepG2 using CL594-67146

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 594-conjugated RANGAP1 Monoclonal antibody (CL594-67146, Clone:1F3A4)(red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67146 targets RANGAP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RANGAP1 fusion protein Ag26136 相同性解析による交差性が予測される生物種
Full Name Ran GTPase activating protein 1
Calculated molecular weight 64 kDa
Observed molecular weight 64 kDa, 82 kDa
GenBank accession numberBC014044
Gene symbol RANGAP1
Gene ID (NCBI) 5905
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission maxima wavelengths594 nm / 615 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.

Protocols

Product Specific Protocols
IF protocol for CL Plus 594 RANGAP1 antibody CL594-67146Download protocol
FC protocol for CL Plus 594 RANGAP1 antibody CL594-67146Download protocol
Standard Protocols
Click here to view our Standard Protocols