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TNFSF11/RANKL Monoclonal antibody

Name: TNFSF11/RANKL Monoclonal antibody
Brand: Proteintech
SKU: 66610-1-Ig

TNFSF11/RANKL Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

3F2E1

Review Product

Cat no : 66610-1-Ig

Synonyms

TNFSF11, TNF-related activation-induced cytokine, Receptor activator of nuclear factor kappa-B ligand, CD254, CD 254

Validation Data Gallery

Filter:

AllWBIF/ICC

WB analysis using 66610-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 66610-1-Ig
WB result of RANKL antibody (66610-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANKL transfected HeLa cells.
WB analysis of DC2.4 using 66610-1-Ig
DC2.4 cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 66610-1-Ig
human spleen tissue were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 66610-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of U2OS using 66610-1-Ig
U2OS cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HUVEC using 66610-1-Ig
HUVEC cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of NCCIT using 66610-1-Ig
NCCIT cell were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IF Staining of MCF-7 using 66610-1-Ig
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RANKL antibody (66610-1-Ig, Clone: 3F2E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Cat No. 66610-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	COLO 320 cells, HeLa cells, U2OS cells, HUVEC cells, NCCIT cells, human spleen tissue, DC2.4 cells, JAR cells, RAW 264.7 cells
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IHC	See 4 publications below
IF	See 3 publications below

Product Information

66610-1-Ig targets TNFSF11/RANKL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	TNFSF11/RANKL fusion protein Ag19975 相同性解析による交差性が予測される生物種
Full Name	tumor necrosis factor (ligand) superfamily, member 11
Calculated molecular weight	317 aa, 35 kDa
Observed molecular weight	35-38 kDa
GenBank accession number	BC074890
Gene symbol	RANKL
Gene ID (NCBI)	8600
RRID	AB_2881970
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.

Protocols

Product Specific Protocols
WB protocol for TNFSF11/RANKL antibody 66610-1-Ig	Download protocol
IF protocol for TNFSF11/RANKL antibody 66610-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	J Cancer Gut microbiota deficiency ameliorates multiple myeloma and myeloma-related bone disease by Th17 cells in mice models Authors - Jia Liu View Article
human	WB, IF	Cell Death Dis RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis. Authors - Qian Liang View Article
mouse	IHC,IF,WB	Open Life Sci Effect of periostin on bone metabolic and autophagy factors during tooth eruption in mice Authors - Han Qin View Article
mouse	WB	J Periodontal Res Role of Piezo1 in modulating the RANKL/OPG ratio in mouse osteoblast cells exposed to Porphyromonas gingivalis lipopolysaccharide and mechanical stress Authors - Mabuki Uchinuma View Article
mouse	IHC,WB	Cell Metab Gut microbial alterations in arginine metabolism determine bone mechanical adaptation Authors - Dan Wang View Article
human	WB	Mediators Inflamm Sorbitol Destroyed Intestinal Microfold Cells (M Cells) Development through Inhibition of PDE4-Mediated RANKL Expression Authors - Li Xiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNFSF11/RANKL Monoclonal antibody

TNFSF11/RANKL Monoclonal Antibody for WB, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 66610-1-Ig

WB analysis of HeLa using 66610-1-Ig

WB analysis of DC2.4 using 66610-1-Ig

WB analysis of human spleen using 66610-1-Ig

WB analysis using 66610-1-Ig

WB analysis of U2OS using 66610-1-Ig

WB analysis of HUVEC using 66610-1-Ig

WB analysis of NCCIT using 66610-1-Ig

IF Staining of MCF-7 using 66610-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Gut microbiota deficiency ameliorates multiple myeloma and myeloma-related bone disease by Th17 cells in mice models

RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis.

Effect of periostin on bone metabolic and autophagy factors during tooth eruption in mice

Role of Piezo1 in modulating the RANKL/OPG ratio in mouse osteoblast cells exposed to Porphyromonas gingivalis lipopolysaccharide and mechanical stress

Gut microbial alterations in arginine metabolism determine bone mechanical adaptation

Sorbitol Destroyed Intestinal Microfold Cells (M Cells) Development through Inhibition of PDE4-Mediated RANKL Expression