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  • Featured Product
  • KD/KO Validated

TNFSF11/RANKL Monoclonal antibody, PBS Only (Capture)

TNFSF11/RANKL Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F2E1

Cat no : 66610-1-PBS

Synonyms

TNFSF11, TNF-related activation-induced cytokine, Receptor activator of nuclear factor kappa-B ligand, CD254, CD 254

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFSF11/RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis using 66610-1-Ig (same clone as 66610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TNFSF11/RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of HeLa using 66610-1-Ig (same clone as 66610-1-PBS)

    WB result of RANKL antibody (66610-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANKL transfected HeLa cells. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DC2.4 cells using TNFSF11/RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of DC2.4 using 66610-1-Ig (same clone as 66610-1-PBS)

    DC2.4 cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using TNFSF11/RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of NCCIT using 66610-1-Ig (same clone as 66610-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using TNFSF11/RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of human spleen using 66610-1-Ig (same clone as 66610-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TNFSF11/RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis using 66610-1-Ig (same clone as 66610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using TNFSF11/RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of U2OS using 66610-1-Ig (same clone as 66610-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using TNFSF11/RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of HUVEC using 66610-1-Ig (same clone as 66610-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TNFSF11/RANKL Monoclonal antibody (66610-1-Ig)

    IF Staining of MCF-7 using 66610-1-Ig (same clone as 66610-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RANKL antibody (66610-1-Ig, Clone: 3F2E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50526-1

    Cytometric bead array standard curve of MP50526-1

    Cytometric bead array standard curve of MP50526-1, RANKL Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66610-1-PBS. Detection antibody: 66610-2-PBS. Standard:Ag19975. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66610-1-PBS targets TNFSF11/RANKL as part of a matched antibody pair:

MP50526-1: 66610-1-PBS capture and 66610-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TNFSF11/RANKL fusion protein Ag19975 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 11
Calculated molecular weight 317 aa, 35 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC074890
Gene symbol RANKL
Gene ID (NCBI) 8600
RRIDAB_2881970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.