• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

RANKL Monoclonal antibody, PBS Only

RANKL Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F2E1

Cat no : 66610-1-PBS

Synonyms

CD254, hRANKL2, ODF, OPGL, OPTB2, Osteoprotegerin ligand, RANKL, sOdf, TNFSF11, TRANCE

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis using 66610-1-Ig (same clone as 66610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of HeLa using 66610-1-Ig (same clone as 66610-1-PBS)

    WB result of RANKL antibody (66610-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANKL transfected HeLa cells. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DC2.4 cells using RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of DC2.4 using 66610-1-Ig (same clone as 66610-1-PBS)

    DC2.4 cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of NCCIT using 66610-1-Ig (same clone as 66610-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of human spleen using 66610-1-Ig (same clone as 66610-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis using 66610-1-Ig (same clone as 66610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of U2OS using 66610-1-Ig (same clone as 66610-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using RANKL Monoclonal antibody (66610-1-Ig)

    WB analysis of HUVEC using 66610-1-Ig (same clone as 66610-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RANKL Monoclonal antibody (66610-1-Ig)

    IF Staining of MCF-7 using 66610-1-Ig (same clone as 66610-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RANKL antibody (66610-1-Ig, Clone: 3F2E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66610-1-PBS targets RANKL in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RANKL fusion protein Ag19975 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 11
Calculated molecular weight 317 aa, 35 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC074890
Gene symbol TNFSF11
Gene ID (NCBI) 8600
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.