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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAP1GAP Polyclonal antibody

RAP1GAP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19174-1-AP

Synonyms

KIAA0474, RAP1 GTPase activating protein, RAP1GA1, RAP1GAP, RAP1GAP1, RAP1GAPII, RAPGAP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAP1GAP Polyclonal antibody (19174-1-AP)

    WB analysis using 19174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19174-1-AP (RAP1GAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAP1GAP Polyclonal antibody (19174-1-AP)

    WB analysis of HeLa using 19174-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19174-1-AP (RAP1GAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RAP1GAP Polyclonal antibody (19174-1-AP)

    WB analysis of Jurkat using 19174-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19174-1-AP (RAP1GAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAP1GAP Polyclonal antibody (19174-1-AP)

    IF Staining of HeLa using 19174-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAP1GAP antibody (19174-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19174-1-AP targets RAP1GAP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAP1GAP fusion protein Ag5576 相同性解析による交差性が予測される生物種
Full Name RAP1 GTPase activating protein
Calculated molecular weight 73 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC054490
Gene symbol RAP1GAP
Gene ID (NCBI) 5909
RRIDAB_2177291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAP1GAP antibody 19174-1-APDownload protocol
IF protocol for RAP1GAP antibody 19174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols