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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RAP1GAP Polyclonal antibody

RAP1GAP Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19174

Synonyms

RAPGAP, RAP1GAP1, RAP1GA1, Rap1 GTPase-activating protein 1, RAP1 GTPase activating protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated RAP1GAP Polyclonal a (CL488-19174)

    IF Staining of HeLa using CL488-19174

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RAP1GAP antibody (CL488-19174) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated RAP1GAP Polyclonal a (CL488-19174)

    FC experiment of HeLa using CL488-19174

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Rap1gap Polyclonal Antibody (CL488-19174)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19174 targets RAP1GAP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAP1GAP fusion protein Ag5576 相同性解析による交差性が予測される生物種
Full Name RAP1 GTPase activating protein
Calculated molecular weight 73 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC054490
Gene symbol RAP1GAP
Gene ID (NCBI) 5909
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RAP1GAP antibody CL488-19174Download protocol
FC protocol for CL Plus 488 RAP1GAP antibody CL488-19174Download protocol
Standard Protocols
Click here to view our Standard Protocols