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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAPGEF2 Recombinant antibody, PBS Only

RAPGEF2 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241637G5

Cat no : 84331-4-PBS

Synonyms

PDZ GEF1, NRAPGEP, nRap GEP, KIAA0313, CNrasGEF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAPGEF2 Recombinant antibody (84331-4-RR)

    WB analysis using 84331-4-RR (same clone as 84331-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84331-4-RR (RAPGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RAPGEF2 Recombinant antibody (84331-4-RR)

    IHC staining of mouse kidney using 84331-4-RR (same clone as 84331-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84331-4-RR (RAPGEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using RAPGEF2 Recombinant antibody (84331-4-RR)

    IF Staining of Jurkat using 84331-4-RR (same clone as 84331-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RAPGEF2 antibody (84331-4-RR, Clone: 241637G5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84331-4-RR

    Affinity and Kinetic Characterization of 84331-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84331-4-RR against Human RAPGEF2 were performed. The affinity constant is 0.19 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84331-4-PBS targets RAPGEF2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAPGEF2 fusion protein Ag35288 相同性解析による交差性が予測される生物種
Full Name Rap guanine nucleotide exchange factor (GEF) 2
Calculated molecular weight167kDa
Observed molecular weight200 kDa
GenBank accession numberNM_014247.2
Gene symbol RAPGEF2
Gene ID (NCBI) 9693
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.