Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, SH-SY5Y cells, Jurkat cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84331-4-RR targets RAPGEF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | RAPGEF2 fusion protein Ag35288 相同性解析による交差性が予測される生物種 |
| Full Name | Rap guanine nucleotide exchange factor (GEF) 2 |
| Calculated molecular weight | 167kDa |
| Observed molecular weight | 200 kDa |
| GenBank accession number | NM_014247.2 |
| Gene Symbol | RAPGEF2 |
| Gene ID (NCBI) | 9693 |
| RRID | AB_3671872 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9Y4G8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAPGEF2 antibody 84331-4-RR | Download protocol |
| IHC protocol for RAPGEF2 antibody 84331-4-RR | Download protocol |
| IF protocol for RAPGEF2 antibody 84331-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |